Journal: Cell reports

Article Title: Post-translational Regulation of FNIP1 Creates a Rheostat for the Molecular Chaperone Hsp90

doi: 10.1016/j.celrep.2019.01.018

Figure Lengend Snippet:

Article Snippet: Rabbit anti-PP5 , Cell Signaling Technology , Cat# 2289; RRID:AB_2168757.

Techniques: Virus, Recombinant, Mass Spectrometry, Software

Journal: Neurobiology of aging

Article Title: Administration of the benzodiazepine midazolam increases tau phosphorylation in the mouse brain

doi: 10.1016/j.neurobiolaging.2018.10.027

Figure Lengend Snippet: List of antibodies used

Article Snippet: A detailed summary of all of the antibodies used in these studies is shown in . table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Antibody Phospho-Epitope Type Source Manufacturer Location Catalog # WB Dilution Tau Tau46 Total Tau Monoclonal Mouse Cell Signaling Danvers, MA 4019 1:2500 Anti-Human Tau A0024 Total Tau 243-441 Polyclonal Rabbit Dako Cytomation Carpinteria, CA A002401-2 1:10000 TG5 Total Tau 220-240 Monoclonal Mouse Peter Davies Bronx, NY N/A 1:1000 AT8 pSer202/pThr205 Monoclonal Mouse Thermo Scientific Waltham, MA MN1020 1:10000 CP13 pSer202 Monoclonal Mouse Peter Davies Bronx, NY N/A 1:1000 PHF-1 pSer396/pSer404 Monoclonal Mouse Peter Davies Bronx, NY N/A 1:1000 AT180 pThr231 Monoclonal Mouse Thermo Scientific Waltham, MA MN1040 1:1000 PS422 pSer422 Polyclonal Rabbit Life Technologies Carlsbad, CA 44-764G 1:1000 PS262 pSer262 Polyclonal Rabbit Life Technologies Carlsbad, CA 44-750G 1:1000 PS199 pSer199 Polyclonal Rabbit Life Technologies Carlsbad, CA 44-734G 1:1000 AT270 pThr181 Monoclonal Mouse Thermo Scientific Waltham, MA MN1050 1:1000 12 E8 pSer262/pSer356 Monoclonal Mouse Pharmaceuticals Francisco, CA N/A 1:1000 Kinases GSK-3β Monoclonal Rabbit Cell Signaling Danvers, MA 9315 1:1000 Phospho-GSK-3 β (Ser9) Ser9 Polyclonal Rabbit Cell Signaling Danvers, MA 9336 1:1000 SAPK/JNK Monoclonal Rabbit Cell Signaling Danvers, MA 9258 1:1000 Phospho-SAPK/JNK Thr183/Tyr185 Monoclonal Rabbit Cell Signaling Danvers, MA 4671 1:1000 p44/42 MAPK Monoclonal Mouse Cell Signaling Danvers, MA 9107 1:2000 Phospho-p44/42 MAPK Thr202/Tyr204 Monoclonal Mouse Cell Signaling Danvers, MA 9106 1:2000 CaMKII Polyclonal Rabbit Cell Signaling Danvers, MA 3362 1:1000 Phospho-CaMKII Thr286 Polyclonal Rabbit Cell Signaling Danvers, MA 3361 1:1000 p35/25 Monoclonal Rabbit Cell Signaling Danvers, MA 2680 1:1000 Phosphatases PP2A-C (catalytic subunit) Monoclonal Mouse Sigma-Aldrich St. Louis, MO A5316 1:10000 demethylated PP2A-C Monoclonal Mouse Cell Signaling Danvers, MA 4466 1:1000 PP1 (E-9) Monoclonal Mouse Santa Cruz Dallas, TX sc-7482 1:1000 Pan-Calcineurin A (PP2B) Polyclonal Rabbit Cell Signaling Danvers, MA 2614 1:1000 PP5 Polyclonal Rabbit Cell Signaling Danvers, MA 2289 1:1000 Gel loading Controls β-actin Monoclonal Mouse Sigma-Aldrich St.Louis, MO A5316 1:10000 β3-tubulin Monoclonal Mouse Cell Signaling Danvers, MA 4466 1:1000 Open in a separate window List of antibodies used 2.3.

Techniques:

Journal: Neurobiology of aging

Article Title: Administration of the benzodiazepine midazolam increases tau phosphorylation in the mouse brain

doi: 10.1016/j.neurobiolaging.2018.10.027

Figure Lengend Snippet: Cortical proteins from the in 8-10 week old C57BL/6 mice that received either saline (Ctl) or midazolam 25 mg/kg i.p. and were sacrificed 30 min (Mid 0.5) or 24h (Mid 24) were separated by SDS-PAGE and protein levels of several protein phosphatases were determined using antibodies directed at (A) the demethylated protein phosphatase catalytic subunit (dm-PP2A-C), (B) the PP2A catalytic subunit (PP2A-C), (C) protein phosphatase 1 (PP1), (D) protein phosphatase 2B (PP2B), and (E) protein phosphatase 5 (PP5). Relative immunoreactive band intensities are expressed as a percent of saline control (Ctl) after appropriately normalizing for gel loading with β-actin or β-tubulin. For each condition, 2 representative immunoblot bands are displayed with Ctl (n = 6), 0.5h (n = 6), and 24h (n = 6). Data are expressed as mean ± SD. * denotes P < 0.05 vs. Ctl using ANOVA with Tukey’s post hoc test.

Article Snippet: A detailed summary of all of the antibodies used in these studies is shown in . table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Antibody Phospho-Epitope Type Source Manufacturer Location Catalog # WB Dilution Tau Tau46 Total Tau Monoclonal Mouse Cell Signaling Danvers, MA 4019 1:2500 Anti-Human Tau A0024 Total Tau 243-441 Polyclonal Rabbit Dako Cytomation Carpinteria, CA A002401-2 1:10000 TG5 Total Tau 220-240 Monoclonal Mouse Peter Davies Bronx, NY N/A 1:1000 AT8 pSer202/pThr205 Monoclonal Mouse Thermo Scientific Waltham, MA MN1020 1:10000 CP13 pSer202 Monoclonal Mouse Peter Davies Bronx, NY N/A 1:1000 PHF-1 pSer396/pSer404 Monoclonal Mouse Peter Davies Bronx, NY N/A 1:1000 AT180 pThr231 Monoclonal Mouse Thermo Scientific Waltham, MA MN1040 1:1000 PS422 pSer422 Polyclonal Rabbit Life Technologies Carlsbad, CA 44-764G 1:1000 PS262 pSer262 Polyclonal Rabbit Life Technologies Carlsbad, CA 44-750G 1:1000 PS199 pSer199 Polyclonal Rabbit Life Technologies Carlsbad, CA 44-734G 1:1000 AT270 pThr181 Monoclonal Mouse Thermo Scientific Waltham, MA MN1050 1:1000 12 E8 pSer262/pSer356 Monoclonal Mouse Pharmaceuticals Francisco, CA N/A 1:1000 Kinases GSK-3β Monoclonal Rabbit Cell Signaling Danvers, MA 9315 1:1000 Phospho-GSK-3 β (Ser9) Ser9 Polyclonal Rabbit Cell Signaling Danvers, MA 9336 1:1000 SAPK/JNK Monoclonal Rabbit Cell Signaling Danvers, MA 9258 1:1000 Phospho-SAPK/JNK Thr183/Tyr185 Monoclonal Rabbit Cell Signaling Danvers, MA 4671 1:1000 p44/42 MAPK Monoclonal Mouse Cell Signaling Danvers, MA 9107 1:2000 Phospho-p44/42 MAPK Thr202/Tyr204 Monoclonal Mouse Cell Signaling Danvers, MA 9106 1:2000 CaMKII Polyclonal Rabbit Cell Signaling Danvers, MA 3362 1:1000 Phospho-CaMKII Thr286 Polyclonal Rabbit Cell Signaling Danvers, MA 3361 1:1000 p35/25 Monoclonal Rabbit Cell Signaling Danvers, MA 2680 1:1000 Phosphatases PP2A-C (catalytic subunit) Monoclonal Mouse Sigma-Aldrich St. Louis, MO A5316 1:10000 demethylated PP2A-C Monoclonal Mouse Cell Signaling Danvers, MA 4466 1:1000 PP1 (E-9) Monoclonal Mouse Santa Cruz Dallas, TX sc-7482 1:1000 Pan-Calcineurin A (PP2B) Polyclonal Rabbit Cell Signaling Danvers, MA 2614 1:1000 PP5 Polyclonal Rabbit Cell Signaling Danvers, MA 2289 1:1000 Gel loading Controls β-actin Monoclonal Mouse Sigma-Aldrich St.Louis, MO A5316 1:10000 β3-tubulin Monoclonal Mouse Cell Signaling Danvers, MA 4466 1:1000 Open in a separate window List of antibodies used 2.3.

Techniques: Saline, SDS Page, Control, Western Blot

Journal: Shock

Article Title: Knockdown of TFPI-Anchored Endothelial Cells Exacerbates Lipopolysaccharide-Induced Acute Lung Injury Via NF-κB Signaling Pathway

doi: 10.1097/shk.0000000000001120

Figure Lengend Snippet: Figure 2.Coagulative function and TFPI concentration in TFPI cKO mice with ALI . WT

Article Snippet: Goat anti-mouse ACE2 and TFPI polyclonal antibodies were obtained from R&D Systems.

Techniques: Concentration Assay

Journal: Shock

Article Title: Knockdown of TFPI-Anchored Endothelial Cells Exacerbates Lipopolysaccharide-Induced Acute Lung Injury Via NF-κB Signaling Pathway

doi: 10.1097/shk.0000000000001120

Figure Lengend Snippet: Figure3.Endothelial TFPI deletion exacerbated lung pathological changes in LPS-induced

Article Snippet: Goat anti-mouse ACE2 and TFPI polyclonal antibodies were obtained from R&D Systems.

Techniques:

Journal: Shock

Article Title: Knockdown of TFPI-Anchored Endothelial Cells Exacerbates Lipopolysaccharide-Induced Acute Lung Injury Via NF-κB Signaling Pathway

doi: 10.1097/shk.0000000000001120

Figure Lengend Snippet: Figure4. Endothelial TFPI deletion increased vascular permeability in acute lung injury.(A)

Article Snippet: Goat anti-mouse ACE2 and TFPI polyclonal antibodies were obtained from R&D Systems.

Techniques: Permeability

Journal: Shock

Article Title: Knockdown of TFPI-Anchored Endothelial Cells Exacerbates Lipopolysaccharide-Induced Acute Lung Injury Via NF-κB Signaling Pathway

doi: 10.1097/shk.0000000000001120

Figure Lengend Snippet: Figure5. Accumulation of inflammatory cells and lung inflammatory response were enhanced in TFPIcKO mice following ALI. To confirm that the inflammatory response was aggravated in TFPI cKO mice compared with WT mice, we measured cell number and cytokines

Article Snippet: Goat anti-mouse ACE2 and TFPI polyclonal antibodies were obtained from R&D Systems.

Techniques:

Journal: Shock

Article Title: Knockdown of TFPI-Anchored Endothelial Cells Exacerbates Lipopolysaccharide-Induced Acute Lung Injury Via NF-κB Signaling Pathway

doi: 10.1097/shk.0000000000001120

Figure Lengend Snippet: Figure6. Endothelial-specific disruption of TFPI destroyed endothelial gap by activation of

Article Snippet: Goat anti-mouse ACE2 and TFPI polyclonal antibodies were obtained from R&D Systems.

Techniques: Disruption, Activation Assay

Journal: Journal of thrombosis and haemostasis : JTH

Article Title: Tissue factor Deficiency Increases Alveolar Hemorrhage and Death in Influenza A Infected Mice

doi: 10.1111/jth.13307

Figure Lengend Snippet: We analyzed TF antigen expression in the lungs of uninfected (B, E) and infected (7 dpi) wild-type mice (C, F), and infected TFΔEp mice (G–I) by immunohistochemistry. Tissue sections were incubated with (B, C, E, F–I) or without (A and D) a goat anti-mouse TF polyclonal antibody. The black arrows indicate TF expression in the epithelium of bronchi (Bc). The arrowheads indicate TF staining in adventitial cells of blood vessels (Bv). The white arrow indicates TF expression in adventitial cells surrounding a bronchiole. Original magnification x200. Scale bar is 100 µm.

Article Snippet: Then, slides were incubated overnight at 4°C with 1 μg/mL anti-human TF goat polyclonal antibody (R&D Systems, Minneapolis, MN), rinsed and incubated with 1.5 μg/mL biotinylated rabbit anti-goat secondary antibody (Vector Lab) for 20 min at room temperature.

Techniques: Expressing, Infection, Immunohistochemistry, Incubation, Staining